WARF reference number P00315US describes a method of using traceless Staudinger ligation for the total chemical synthesis of proteins. Publications Tam A., Soellner M.B. and Raines R.T. 2007. Water-Soluble Phosphinothiols for Traceless Staudinger Ligation and Integration with Expressed Protein Ligation. J. Am. Chem. Soc. 129, 11421–11430.
Jan 13, 2020 Traceless synthesis of ceramides in living cells reveals Jul 17, 2018 Long‐Range Chemical Ligation from N→N Acyl Migrations in Abstract Chemical ligations to form native peptides from N→N acyl migrations in Trp‐containing peptides via 10‐ to 18‐membered cyclic transition states are described. In this study, a statistical, (PDF) Traceless chemical ligations from O-acyl serine sites
The Staudinger-Bertozzi ligation [1]: Bertozzi developed the Staudinger-then-aza-Wittig reaction shown below and demonstrated its utility in ligating large phosphorescent compounds by the amide bond. This reaction is high yielding and highly chemoselective, offering possibilities for applications in various chemical biology researches.
Martin Köckerling, Constantin Mamat, Structural and Kinetic Considerations for the Application of the Traceless Staudinger Ligation to Future 18F Radiolabeling Using XRD and 19F NMR, International Journal of Chemical Kinetics, 10.1002/kin.21137, 50, 1, (31-40), (2017).
Notable attributes of this ligation strategy include its ability to generate an amide product without either residual atoms, 3 or racemization, 4 and a high reaction rate. 5 (Diphenylphosphino)methanethiol (1) is the most often used of known reagents for effecting the traceless Staudinger ligation, and has mediated the orthogonal assembly of a
Convergent Glycopeptide Synthesis by Traceless Staudinger An approach combining traceless Staudinger ligation and protease‐catalyzed N‐terminal azidonation has been shown to be efficient for the convergent synthesis of glycopeptides without the cysteine limitation of native chemical ligation. Traceless Chemical Ligation from S-, O-, and N-Acyl This “fragment condensation” has been achieved in many ways both in solution and by solid-phase peptide synthesis (SPSS) on resin. The most successful method for in-solution coupling is known as native chemical ligation (NCL), and the technique dates back to the pioneering work of Wieland (1953) and subsequently Kent (1994) among many others.